Folpet Analysis on Primesep 100 and Obelisc R HPLC Columns

  Folpet is a fungicide which is used to prevent a variety of fungal infections such as apple scab, cherry leaf spot, rose black spot, and rose mildew. Folpet was one of many pesticides studied by the EURL-SRM, a proficiency test for single residue methods developed by the European Union Reference Laboratory. Folpet was retained and separated from impurities with Obelisc R and Primesep 100 columns. Primesep 100 contains embedded acidic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method can be used for dozens of other pesticides and is LC/MS compatible.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Folpet
 

Application Analytes:

Folpet