HILIC Separation of Sugars on Obelisc N

Common sugars can be separated on an Obelisc N HILIC/cation-exchange column with LC/MS compatible mobile phase. Highly polar surface of Obelisc N column provides good retention for sorbitol, sucrose, lactose and raffinose. Other sugars can be separated using this column.

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Hydrophilic, Ionizable, Sugars
Analyzing Compounds Sorbitol, Sucrose, Lactose,  Raffinose


Application Analytes:

HILIC Separation of Sugars on Obelisc N