HILIC Separation of Surfactant Triton X-100

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)


Column Obelisc N,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection UV 250, 230nm


Class of Compounds Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100

Application Analytes:

Triton X100