HPLC Analysis of 2,4,5-T on Mixed-Mode Columns

  2,4,5-T is a synthetic herbicide that defoliates broad-leaf plants. It was a major component of Agent Orange, a defoliant used during the Vietnam War. 2,4,5-T has been replaced by dicamba and triclopyr due to its possible toxicity. 2,4,5-T was included in a proficiency test ran by the EURL (European Union Reference Laboratory) using QuEChERS methodology on acidic pesticides. Obelisc R retained 2,4,5-T because it has a long hydrophobic chain and ionic pairing groups. Primesep 100 retains by reverse-phase and with acid ion-pairing groups. Method is LC/MS compatible and same conditions can be used on many different pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4,5-T
 

Application Analytes:

2,4,5-Trichlorophenoxyacetic acid