HPLC Analysis of ACE Serum Active Ingredients

Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.) retinol and Vitamin E Acetate


Column Primesep  SB , 3.0x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Acetic Acid
Flow Rate 0.5 ml/min
Detection UV 270, 325


Class of Compounds Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Ascorbic acid (Vitamin C), Retinol and Vitamin E Acetate

Application Analytes:

Ascorbic Acid
Vitamin E Acetate
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