HPLC Analysis of Azide Ion on Obelisc N Column

Sodium azide is used in older car airbag systems and airplane escape slides. It is an acutely toxic substance. Obelisc N mixed-mode column can retain the azide ion using MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate as buffer. Can also be UV detected at 250nm.


Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 85/15%
Buffer AmAc pH 5- 10 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Anion, Hydrophilic, Ionizable
Analyzing Compounds Azide

Application Analytes:

Azide Ion