HPLC Analysis of Bentazon on Primesep and Obelisc Mixed-Mode Columns

Bentazon is a thiadiazine herbicide which is used to control the growth of specific plants. Some plants are not affected such as alfalfa, corn and peanuts. The EURL (European Union Reference Laboratory) included bentazon in an analysis of acidic pesticides using QuEChERS method. Primesep 100 and Obelisc R were used to retain and separate bentazon from impurities. Method is LC/MS compatible and developed to be used for many pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Bentazon
 

Application Analytes:

Bentazon