HPLC Analysis of Citrulline and Arginine on Primesep Columns

Two underivatized amino acids, citrulline and arginine, are separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns with LC/MS compatible mobile phase. Method can be used for retention and separation of underivatized amino acids without ion-pairing reagent in the mobile phase.

Condition

Column Primesep, 2.1x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 0.2 ml/min
Detection ELSD
 

Description

Class of Compounds Ions,  Hydrophilic, Ionizable
Analyzing Compounds Sodium, Citrulline, Arginine, Chloride


Application Analytes:

Arginine
Citrulline