HPLC Analysis of Dichloran on Mixed-Mode Columns

  Dichloran (DCNA) is a fungicide analyzed on the mixed-mode columns Obelisc R and Primesep 100. DCNA was separated from impurities on Obelisc R, which contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Primesep 100 which contains embedded acidic ion-pairing groups also retained DCNA. Method is LC/MS compatible and can be used as a general approach for analyzing DCNA and many other pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%
Buffer Gradient AmAc pH 3.0- 30-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Hydrophobic, Ionizable
Analyzing Compounds Dichloran
 

Application Analytes:

Dichloran