HPLC Analysis of Polar Basic and Acetic Compounds on Primesep AB Column

Mixture of polar acidic and basic compounds is separated on a Primesep AB mixed-mode HPLC column. Dopamine and tyrosine are retained by combination of reversed-phase and cation-exchange mechanisms. Maleic acid is retained by anion-exchange mechanism, and benzoic acid is retained by reversed-phase mechanism. Primesep AB is a trimodal column with a C12 hydrophobic chain and cation-exchange and anion exchange groups on the surface. Method utilizes UV detection but can be used with other detection techniques (ELSD, LC/MS, Corona).


Column Primesep AB, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Tyrosine, Dopamine, Maleic Acid, Benzoic Acid

Application Analytes:

Benzoic Acid
Maleic Acid