HPLC Separation of 2-Naphthoxyacetic acid

 
2-Naphthoxyacetic acid was included in a EURL (European Union Reference Laboratory) report for using QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) single residue methods. Primesep 100 & Obelisc R were used to separate 2-Naphthoxyacetic acid. Primesep 100 is a mixed-mode column which uses embedded ion-pairing groups and Obelisc can fine tune separations using both a long hydrophobic chain and ionic groups. Method is LC/MS compatible and can be used for many pesticides.
 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 3 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Synthetic plant hormone, Acid,   Hydrophobic, Ionizable
Analyzing Compounds 2-Naphthoxyacetic acid
 

Application Analytes:

2-Naphthoxyacetic acid