HPLC Separation of Active Compounds in Drug Formulation

HPLC Separation of Active Compounds in Drug Formulation
An HPLC method for the separation of active drug compounds on a Primesep 200 column. The retention of compounds is achieved through reverse-phase, cation exchange and hydrophobic interactions. Benzoic acid, hyoscyamine sulfate and phenyl salicylate are baseline separated using simple mobile phases of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with a UV detector.

Condition

Column Primesep 200, 3.0x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 220 nm
 

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Benzoic acid, Hyoscyamine Sulfate, Phenylsalicylate)
 

Application Analytes:

Benzoic Acid
Hyoscyamine Sulfate
Phenylsalicylate