Amino acids can be retained and separated on a Primesep S2 column in HILIC/cation-exchange mode.
| Column | Primesep S2, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Dopa, Tyrosine, Phenylalanine |
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Phenylalanine
Tyrosine