HPLC Separation of Amino Acids in HILIC and Cation-Exchange Modes

chr_306.gif Amino acids can be retained and separated on a Primesep S2 column in HILIC/cation-exchange mode.

Condition

Column Primesep S2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Dopa, Tyrosine, Phenylalanine
 

Application Analytes:

DOPA (3,4-dihydroxy-L-phenylalanine)
Phenylalanine
Tyrosine