HPLC Separation of Aminobiphenyl Isomers in Cation-Exchange Mode

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Two isomers of aminobiphenyl were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.

Condition
Column Primesep S2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmAc pH 5.0 15 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description
Class of Compounds
 Hydrophobic, Ionizable
Analyzing Compounds 2-Aminobiphenyl, 4 -Aminobiphenyl

Application Column

Primesep S2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Aminobiphenyl
4-Aminobiphenyl
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.