HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80

chr_288.gif Barium ion and citric acid are analyzed in the presence of sucrose and Tween 80 on Obelisc N HILIC mixed-mode column. Both compounds are retained in ion-exchange mode and HILIC is not employed with this method. Obelisc N HPLC column has basic and acidic ion-exchange groups separated by long hydrophilic chain. Presence of that long chain allows both cation- and anion-exchange mechanisms to exist simultaneously. Obelisc N column can be used for analysis of cation and anions in HILIC/ion-exchange mode and in ion-exchange mode. Analytes can be monitored by ELSD/CAD and LC/MS.  


Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds  Acid, Ion,  Hydrophobic, Ionizable
Analyzing Compounds Barium ion, Citric acid,  Sucrose, Tween 80

Application Analytes:

Citric Acid
Tween 80