HPLC Separation of Benzenesulfonamide and Sulfanilamide

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Condition 1

Column Newcrom А, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeOH/H2O - 80/20%
Buffer H2SO4 - 0.005%
Flow Rate 0.5 ml/min
Detection  275 nm

Condition 2

Column Primesep D, , Primesep 100 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer Formic Acid - 0.1%
Flow Rate 0.5 ml/min
Detection UV 230 nm
Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.

Application Analytes:

Benzenesulfonamide
Sulfanilamide