HPLC Separation of Benzenesulfonic and p-Toluenesulfonic Acids

HPLC.cloud View on hplc.cloud

Condition

Column Newcrom BH, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer H3PO4 - 3%
Flow Rate 0.5 ml/min
Detection UV 275nm

Description

Class of Compounds Acid
Analyzing Compounds Benzenesulfonic, p-Toluenesulfonic Acids
    Benzenesulfonic and toluenesulfonic acids are organic acids used in pharmaceutical industry as counter-ions for basic drugs. Both acids are hydrophilic in nature with strong acidic properties. Obtaining separation, retention and good peak shape with traditional reversed-phase columns is a challenging task. We developed robust mixed-mode approach for separation of benzenesulfonic acid and p-toluenesulfonic acid on a Primesep D reversed-phase anion-exchange column. Elution of these compounds is controlled by the amount of ions in the mobile phase and amount of acetonitrile. Both compounds are separated within 4 minutes and can be monitored by UV, ELSD, CAD or LC/MS. This column can be also used to analyze hydrophobic basic drugs by reversed-phase cation-exclusion mechanisms.

Condition

Column Primesep D , 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc 
Flow Rate 1.0 ml/min
Detection UV 270 nm
 

Description

Class of Compounds Acid,  Hydrophobic
Analyzing Compounds Benzenesulfonic Acid,  p-Toluenesulfonic Acid


Application Analytes:

Benzenesulfonic Acid
Organic Acids
p-Toluenesulfonic Acid (PTSA)
LinkedIn
Share
Instagram
Follow by Email