HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions

Creatine, creatinine, and histidine are all important amino acids in the human body. All of these can be separated by liquid chromatography. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Condition

Column Primesep 200, 3.0x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Formic Acid - 0.1%
Flow Rate 0.5 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Creatine, Creatinine, Histidine
 

Application Analytes:

Creatine
Creatinine
Histidine