HPLC Separation of Cyclodextrin Sulfobutyl Ether and Hydrophobic Compound

chr_294.gif Cyclodextrin sulfobutyl ether is sulfonated derivative of cyclodextrin. The degree of sulfobutylation varies, and the compound is a mixture of several components. All compounds are very polar and acidic in nature, and are not retained by traditional reversed-phase chromatography. Separation was achieved on an Obelisc N column in anion-exchange mode. Hydrophobic naphthalene was used a a reference standard. Cyclodextrin was monitored by ELSD.


Column Obelisc N2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH, THF
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD, 60C UV 270 nm


Class of Compounds Hydrophobic, Ionizable
Analyzing Compounds Sodium, Beta Cyclodextrin Sulfobutyl Ether, Naphthalene

Application Analytes:

Betadex Sulfobutyl Ether Sodium