HPLC Separation of Dopamine and Epinephrine in HILIC and Cation-Exchange Modes

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Dopamine and epinephrine were separated on a Primesep S2 HILIC mixed-mode column. Dopamine and epinephrine are retained by HILIC and cation-exchange mechanisms. Retention time is controlled by the amount of ACN, buffer and buffer pH. Method is compatible with LC/MS and can be used for analysis of polar metabolites in biofluids.



Application Analytes:

Dopamine
Epinephrine