HPLC Separation of Dyes

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Condition 1

Column Newcrom B, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer H3PO4 - 0.2%
Flow Rate 0.5 ml/min
Detection UV, 275 nm
  Various dyes are widely used in food, textile, and pharmaceutical industries. Dies are aromatic compounds containing ionizable groups. Methylene blue, crystal violet lactone, and crystal violet were separated on a Primesep D HPLC column. Method can be used for separation of other dyes and pigments that are basic and hydrophobic in nature.

Condition  2

Column Primesep D, 4.6x50 mm, 3 µm, 100A
Mobile Phase MeCN
Buffer H3PO4 - 0.2%
Flow Rate 0.5 ml/min
Detection UV, 300 nm
 

Description

Class of Compounds Dye, Hydrophilic, Ionizable
Analyzing Compounds Methylene Blue, Crystal violet lactone, Crystal violet
 

Application Analytes:

Crystal Violet
Crystal Violet Lactone
Methylene Blue