HPLC Separation of Epinephrine and Epinephrine Sulfonate

Epinephrine and epinephrine sulfonate are separated on combination of Primesep 100 and Primesep SB HPLC columns. Primesep 100 column retains epinephrine by combination of reversed-phase and cation-exchange mechanisms. Epinephrine sulfonate is retained by weak reversed-phase mechanism. Due to strong zwitter-ionic nature of epinephrine sulfonate, ionic interaction is not available for this molecule. Compounds can be monitored by UV, ELSD, CAD or LC/MS.

Condition

Column Primesep SB, Primesep 100 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 70/30%
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Epinephrine, Epinephrine Sulfonate
 

Application Analytes:

Epinephrine
Epinephrine Sulfonate