HPLC Separation of Estrone

  Estrone, also known at oestrone and E1 is a naturally occurring estrogen which is the predominant estrogen in postmenopausal women. It can be found in its long-lived form estrone sulfate. In certain scenarios estrone can be a carcinogen and causes breast pain, nausea, headaches and leg cramps. It was separated on both Obelisc R and Primesep 100 which have unique modes of retention. Method is LC/MS compatible and useful for separating a number of pesticides.  

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Hormone,  Hydrophobic, Ionizable
Analyzing Compounds Estrone
 

Application Analytes:

Estrone