HPLC Separation of Glutamic Acid and GABA

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Glutamic acid and GABA are neurotransmitters. Glutamic acid and GABA are non-essential amino acids. They are hydrophilic and zwitter-ionic in nature . At lower pH, carboxylic acid groups of amino acids are not ionized, making them more hydrophobic and basic. Underivatized glutamic acid and GABA were retained and separated on a Primesep 100 column using ACN/water/TFA mobile phase. Amino acids can be monitored by low UV or ELSD/CAD. Retention is provided by reversed-phase and cation-exchange mechanism. Method can be used for analysis of underivatized amino acids in various matrices including supplements, vitamin and other complex mixtures. various mobile phase can be used with corresponding detection techniques.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer TFA – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 215 nm, ELSD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Glutamic acid, GABA

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Glutamic Acid
Zwitterion
gamma-Aminobutyric Acid (GABA)

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.