HPLC Separation of Glyphosate Reaction Intermediates and Impurities

Glyphosate and intermediates/impurities of production are separated on an Obelisc N HILIC/ion-exchange column by a combination of HILIC and ion-exchange mechanism. Method can be used in analysis of glyphosate, iminodiacetic acid, (N-phosphonomethyl)-imminodiacetic acid, diethanolamine and related impurities in reaction mixtures, waste and ground waters. Detection techniques are LC/MS, ELSD, CAD and UV.


Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Ammonium Formate
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate, IDA, PMIDA, DEA

Application Analytes:

DEA (Diethanolamine)
IDA (Iminodiacetic acid)
PMIDA (Phosphonomethyliminodiacetic acid)