HPLC Separation of Norepinephrine on Legacy L1 Column

Norepinephrine was analyzed based on the United States Pharmacopeia. The Legacy L1 HPLC column is classified as a reversed-phase column according to USP manual. This column can be used for other methods that require this type of column. Legacy L1 can also be used instead of columns from other column manufacturers.

Condition

Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 60/40%
Buffer Sodium heptanesulfonate - 0.22%
Flow Rate 1.0 ml/min
Detection UV, 280 nm
 

Description

Class of Compounds Drug, Neurotransmitter, Hydrophobic, Ionizable
Analyzing Compounds Norepinephrine
 

Application Analytes:

Norephedrine