HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N


Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm. 

Application Analytes:

Adenosine
Cytidine
Cytosine
Guanosine
Uracil
Uridine