HPLC Separation of Nucleobases (2)


Nucleosides consisting of deoxyribose and a nucleobase cannot be separated on a common C18 column. By using a Primesep 200 mixed-mode column with a cation-exchange mechanism, nucleosides: deoxyuridine, deoxyguanosine, deoxycytidine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and TFA as buffer. UV detection at 270nm. 

Application Analytes:

Deoxyadenosine
Deoxycytidine
Deoxyuridine