HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column


The Separation of Phenylalanine, Glucosamine and Tryptophan demonstrates how the small changes to the organic and/or buffer concentrations in the mobile phase affect the retention of compounds on a mixed-mode column by hydrophobic, ion-exchange and ion-exclusion mechanisms in reverse-phase HPLC chromatography. Evaporative Light Scattering Detector (ELSD) used

Condition

Column Primesep 100, 2.1x100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 0.25 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Phenylalanine, Glucosamine, Tryptophan


Application Analytes:

Glucosamine
Phenylalanine
Tryptophan
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