HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column


The Separation of Phenylalanine, Glucosamine and Tryptophan demonstrates how the small changes to the organic and/or buffer concentrations in the mobile phase affect the retention of compounds on a mixed-mode column by hydrophobic, ion-exchange and ion-exclusion mechanisms in reverse-phase HPLC chromatography. Evaporative Light Scattering Detector (ELSD) used 

Application Analytes:

Glucosamine
Phenylalanine
Tryptophan