HPLC Separation of Phosphamidon on Mixed-Mode Columns

  Phosphamidon is an organophosphate insecticide highly toxic to mammals and classified by the World Health Organization to be extremely hazardous (Ia), with an oral lethal dose of less than 5mg/kg of bodyweight. The EURL (European Union Reference Laboratory) included phosphamidon as a target pesticide for the EUPT-CF9. Primesep 100 and Obelisc R were used to retain phosphamidon and separate it from impurities. Primesep 100 contains embedded acidic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing phosphamidon, other organophosphate insecticides, and dozens of other pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Phosphamidon
 

Application Analytes:

Phosphamidon