HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N

Ion chromatography is usually used for analysis of hydrophilic organic and inorganic ions. Same separation can be achieved on HILIC/mixed-mode Obelisc N HPLC columns. Obelisc N HPLC columns have very polar groups on their surface: one of the groups is basic and the other acidic. In case of low organic concentration, two groups are connected by hydrophilic linker. Obelisc N column can be used as cation-exchange and anion-exchange column. This allows to separate positively and negatively charged molecules in one run. Five anions (chloride, bromide, methanesulfonate, nitrate and perchlorate) along with one cation (sodium) were separated in one run. Method is compatible with ELSD, CAD and LC/MS and can be used for analysis of various hydrophilic and hydrophobic cations and anions in one HPLC run.

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Hydrophilic, Ions
Analyzing Compounds Chloride, Nitrate, Chlorate, Bromide, Potassium
 

Application Analytes:

Bromide
Chloride
Methanesulfonic Acid
Nitrate
Nitric Acid
Potassium
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