HPLC Separation of Saccharin and Sorbitol

Sorbitol and saccharin are two sugar substitutes used in food and drinks. Separation of sorbitol and saccharin was achieved on a Primesep N HILIC column using acetonitrile/water with ammonium formate. Compounds were monitored by combination of UV and ELSD. Method is compatible with LC/MS detection.

Application Analytes:

Saccharin
Sorbitol
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