HPLC Separation of Salicylic acid

 

 

 

Salicylic acid is a pain-reliever and anti-inflammatory commonly found in rubefacient products for topical pain relief as well as cosmetic skin-care products used to treat a variety of skin conditions. Legacy L1 was used to retain salicylic acid by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

Condition

Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeOH – 45%
Buffer Acetic acid – 0.55%
Flow Rate 1.0 ml/min
Detection UV, 280 nm

 

 

Description

Class of Compounds
 Acid,  Hydrophilic, Ionizable
Analyzing Compounds Salicylic acid

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

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Application Analytes:
Salicylic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.