Five sugars were separated on a Primesep S2 HILIC column with LC/MS compatible conditions. Various mobile phase produce different selectivity and separation. Method can be used as alternative to aminopropyl column. Primesep S2 column is stable and does not undergo rapid hydrolysis like aminopropyl columns.
| Column | Primesep S2, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O -80/20% |
| Buffer | AmAc pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Fructose, Glucose, Sucrose, Lactose, Maltose, Sorbitol, Sucrose, Alfa D-Lactose, Beta D–Lactose, Raffinose |
Application Analytes:
Fructose
Glucose
Lactose
Maltose
Raffinose
Sorbitol
Sucrose
alpha-D-Lactose
beta-D-Lactose