HPLC Separation of Uridine and Uracil

Uracil is a naturally occurring pyrimidine derivative. Uracil is used for drug delivery and as a pharmaceutical. Uridine is a product of uracil and sugar ribose. Uridine is one of components of ribonucleic acid. Uracil and uridine are slightly basic in nature. Both compounds are hydrophilic and uracil is very often is used as a void marker in reversed-phase chromatography. Uracil and uridine are separated based on their polar properties on a Primesep N HILIC column. Uracil and uridine are monitored by UV.


Column Primesep N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 90/10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 250 nm


Class of Compounds Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Uracil, Uridine

Application Analytes: