View on hplc.cloud
| Column | Newcrom B , 3.2x50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 20/80% |
| Buffer | H3PO4 - 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm |
Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.
| Column | Primesep D, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TFA, AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds | Acid, Monocarboxylic acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Vanilmandelic, Hydroxyindoleacetic, Homovanillic Acid |
Application Analytes:
5-Hydroxyindole-3-Acetic Acid
Homovanillic Acid
Vanilmandelic Acid