HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids


Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.

Application Analytes:

5-Hydroxyindole-3-Acetic Acid
Homovanillic Acid
Vanilmandelic Acid