HPLC Separation of Vitamin B

Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.

Condition

Column Primesep C , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O  -10/90%
Buffer NEt3H3PO4 Ph 3.0 - 20 Mm
Flow Rate 1.0 ml/min
Detection UV, 280 nm
 

Description

Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Thiamine, Riboflavin


Application Analytes:

Pyridoxine
Riboflavin (Vitamin B2)
Thiamine (Vitamin B1)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamins
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