HPLC Separation of Vitamin E and Vitamin E Acetate on Primesep B Column

Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.

Condition

Column Primesep B, 3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 90/10%
Buffer
Flow Rate 0.5 ml/min
Detection UV 205

Description

Class of Compounds Vitamins, Hydrophilic, Ionizable
Analyzing Compounds Vitamin E,  Vitamin E Acetate


Application Analytes:

Vitamin E
Vitamin E Acetate
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