HPLC UV Analysis of Copper Ions in Salt Mixture

A unique approach for analysis of copper ions in a high salt content matrix was developed using EDTA as a visualization agent. Since copper ions can form colored complexes with EDTA, this property can be used for selective analysis of copper in the mixture with other cations, which do not form a colored complex with EDTA. Copper ion can be monitored by UV while other metals pass through the column undetected by UV. This method can be used to analyze copper ion at parts per million concentrations.

Condition

Column Primesep SB, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer AmAc, EDTA
Flow Rate 1.0 ml/min
Detection UV, 310 nm

 

Description

Class of Compounds
Ions
Analyzing Compounds Copper

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Copper
Copper Sulfate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.