Hydrophobic and Hydrophilic Compound Separation

Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Nonpolar, Polar, Supplements
Analyzing Compounds Cysteine,  L-cystine, 2,2-dimethylcystine,  2-methylcysteine, Benzoic Acid,  Toluene
 

Application Analytes:

2,2-Dimethylcysteine
2-Methylcysteine
Amino Acids
Benzoic Acid
Cysteine
L-Cystine
Toluene