Improved Peak Shape of a Basic Compound on Primesep B2 Column

Primesep B2 shows superior efficiency, symmetry, and peak shape for a hydrophilic basic compound compared to two leading reversed-phase C18 columns. Primesep B2 contains an embedded basic functional group that shields the underlying silica by ionic repulsion with the analyte. On reversed-phase columns exposed active silanols provide unintended polar interactions with basic analytes that lead to tailing. The HPLC separation uses a simple mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 250 nm.

Condition

Column Primesep B2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Oxymetazoline
 

Application Analytes:

Oxymetazoline