MCPB Analysis Using Obelisc and Primesep Analytical HPLC Columns

 
MCPB (4-(4-Chloro-methylphenoxy)butanoic acid) is an herbicide used on post-emergence broad-leaf weeds. Its acute toxicity is low to moderate, and has been show to damage liver and kidney tissue. Obelisc R and Primesep 100 were both used to separate MCPB. Primesep 100 is a reverse-phase column with embedded acidic ion-pairing groups while Obelisc R is a mixed-mode column with separates using ionic groups and a long hydrophobic chain. The European Union Reference Laboratory (EURL) included MCPB in an analysis of acidic pesticides using single residue methods. Method is LC/MS compatible and useful for many pesticides.
 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds MCPB
 

Application Analytes:

MCPB