Mecoprop Analysis on Primesep and Obelisc Mixed-Mode HPLC Columns

Mecoprop or methylchlorophenoxypropionic acid (MCPP) is a common herbicide found in many weed-killing/fertilizer products. The EURL (European Union Reference Laboratory) placed mecoprop in an Analysis of Acidic Pesticides using QueChERS method. Obelisc R and Primesep 100 were used to retain mecoprop, each with unique selectivity due to multiple modes of separation. Obelisc R contains embedded ion-pairing groups and a long hydrophobic chain, while Primesep 100 contains acidic ion-pairing groups. Method is LC/MS compatible and capable of retaining many different pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Mecoprop
 

Application Analytes:

Mecoprop