Phosphamidon Analysis Using Obelisc and Primesep Mixed-Mode HPLC Columns

 
Phosphamidon is an organophosphate insecticide commercially available as 70% Z-isomer and 30% E-isomer. It is highly toxic to mammals, and is classified by the World Health Organization to be extremely hazardous (Ia), with an oral lethal dose of less than 5mg/kg of bodyweight. The EURL included phosphamidon as a target pesticide for the EUPT-CF9. Primesep B2 and Obelisc R were used to retain phosphamidon and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing phosphamidon, other organophosphate insecticides, and dozens of other pesticides.
 

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Phosphamidon
 

Application Analytes:

Phosphamidon