Primesep C Column Retention Control

Primesep C separates benzylamines with an easily tunable HPLC method that allows retention control. Benzylamine, N-methylbenzylamine, and N,N-dimethylbenzylamine peak order and retention can be significantly changed by altering the water/acetonitrile ratio in the mobile phase which changes the combination of cation exchange, complex formation, and hydrophobic interactions. This mixture is separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 210 nm.

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV 210

Description

Class of Compounds Hydrophilic, Ionizable
Analyzing Compounds Benzylamine, N-methylbenzylamine,  N,N-dimethylbenzylamine


Application Analytes:

Benzylamine
N,N-Dimethylbenzylamine
N-Methylbenzylamine