Retention of Azide Ion on Obelisc N Column

Sodium azide was analyzed on the Obelisc N HILIC/mixed-mode column. Retention time of the azide ion is controlled by the amount of acetonitrile, buffer concentration, and buffer pH. Mechanism of retention is a hydrophilic interaction and an anion exchange. Method utilizes UV detector for HPLC analysis of azide ion.

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