Retention of Taurine on Obelisc N Column

Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the Obelisc N HILIC/mixed-mode column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.

Condition

Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Taurine
 

Application Analytes:

Taurine
Zwitterion