Separating Triadimefon from Impurities on Primesep 100 and Obelisc R HPLC Columns

   

Triadimefon is known as Bayleton, usually used in a mixture of pesticides including captan, and folpet. A triazole family pesticide, triadimefon is a systemic fungicide that controls the mildew and fungal pests on trees, turf and fruits. It is considered moderately toxic and a method is useful for analyzing triadimefon. Primesep 100 and Obelisc R were the mixed-mode columns used. Primesep 100 separates using reverse-phase and acidic ion-pairing groups. Obelisc R uses  a long hydrophobic chain and multiple ion-pairing groups. Method is LC/MS compatible and can be use for dozens of pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Triadimefon
 

Application Analytes:

Triadimefon