Separation and Retention of Dextromethorphan Bromide in Mixed-Mode HPLC: Buffer Concentration Effect

 
Dextromethorphan is used as a temporary cough suppressant in many medications. The elution time of dextromethorphan and bromide can be reversed in HPLC chromatography on a Primesep B2 reverse-phase (RP) column with embedded basic ion-pairing groups by only changing the concentration of buffer in a simple mobile phase of water and acetonitrile (MeCN, ACN). Buffer is trifluoroacetic acid (TFA). UV detection at 210nm. 

Application Analytes:

Bromide
Dextromethorphan