HPLC Separation of Acetylcholines

Primesep C separates acetylcholine and methylacetylcholine from their chloride counter ion with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. The mass spec (LC/MS) compatible method uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD).  


Column Primesep C, 3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc ph 4.1
Flow Rate0.5  ml/min
Detection ELSD


Class of Compounds Drug
Analyzing Compounds Acetylcholine, Methylacetylcholine

Application Analytes: